5/21/2023 0 Comments Last wood igg![]() Many of the proteins secreted in the human body are glycoproteins, such as immunoglobulins. Introduction Glycosylation is a common protein post translation modification, where the structure and functionality of proteins are altered by the presence of glycans. The developed method utilizing wood materials introduced an environmentally friendly option for glycoproteomics, saving both costs and chemicals, while exhibiting high performance. After delignification, binding capacity and recovery could be improved due to increased hydrophilicity and porosity. Native balsa wood without any pretreatment provided a green alternative to a commercial HILIC product, exhibiting comparable, or even better, enrichment performance for IgG standard and human plasma IgG samples. Here, balsa wood, a naturally available hydrophilic material with highly porous structure, was investigated for immunoglobulin G (IgG) glycopeptide enrichment in a micropipette set-up. Many hydrophilic materials have been used for glycopeptide enrichment, however involving complex synthesis procedures, high consumption in cost, time, and chemicals. Glycoproteomics is one of the main routes to study protein post-translational modifications, and enrichment is one of the important steps. E-mail: c KTH Royal Institute of Technology, School of Engineering Sciences in Chemistry, Biotechnology and Health, Department of Chemistry, Division of Organic Chemistry, Stockholm SE-100 44, Sweden d Westlake University, School of Science, Center of Artificial Photosynthesis for Solar Fuels, Hangzhou CN-310024, China E-mail: b KTH Royal Institute of Technology, School of Engineering Sciences in Chemistry, Biotechnology and Health, Department of Fiber and Polymer Technology, Wallenberg Wood Science Center, Stockholm SE-100 44, Sweden. * a a KTH Royal Institute of Technology, School of Engineering Sciences in Chemistry, Biotechnology and Health, Department of Chemistry, Division of Applied Physical Chemistry, Analytical Chemistry, Stockholm SE-100 44, Sweden. Green Chem., 2020, 22, 5666-5676 Enrichment of glycopeptides using environmentally friendly wood materials †
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